p53在肝细胞肝癌和食管鳞癌中的表达

Expression of p53 in Hepatic Cellular Carcinoma and Squamous Cell Carcinoma of Esophagus

目的 通过检测p5 3在肝细胞肝癌和食管鳞癌中的阳性表达 ,探讨p5 3与肝细胞肝癌和食管鳞癌临床病理学之间的关系 ,帮助诊断肿瘤及判断预后。方法 福尔马林固定 ,石蜡包埋 ,免疫组化LSAB法染色 ,免疫组化ABC法检测。结果 肝细胞肝癌中p5 3阳性率为 79.49% ,HBsAg阳性者为 87.93% ,肝硬变者为 90 .14% ,癌转移者为 92 .5 %。食管鳞癌中p5 3阳性率为 5 3.0 1%。癌转移者为 6 3.41%。两种肿瘤p5 3阳性反应均随癌分化程度降低而增强。结论 p5 3在肝细胞肝癌和食管鳞癌中的阳性表达基本平行 ,阳性反应程度与HBsAg、周围病变、癌分化。

Objective To approach the relationship between p53 and clinical pathology in hepatic cellular carcinoma and squamous cell carcinoma of esophageal to help diagnosis and prognosis of tumor through testing the positive expression of p53 in hepatic cellular carcinoma and squamous cell carcinoma of esophagus. Method Formalin fixation,paraffin imbedding,ST in immunohistochemistry LSAB method and testing in immunohistochemistry ABC method. Result The positive rate of p53 in hepatic cellular carcinoma was 79.40 percent and the positive rate of HBsAg was 87.93 percent ,the liver cirrhosis was 90.14 percent and that of cancerometastasis was 92.5 percent. The positive rate of p53 in squamouscellcarcinomaofesophagus was 53.01 percent and that of cancerometastasis was 63.41 percent.The positive reaction of protein p53 increases along with the decrease of division of cancer in the above mentioned two kinds of cancer. Conclusion The positive expression of p53 is basically horizontalis in hepatic cellular carcinoma and spuamous cell carcinoma of esophagus,the degree of positive reaction is in positive correlation with HBsAg,peripheric affection,the division of cancer and cancerometastasis.