CO_2激光对蚕豆诱变效应试验研究

INDUCED MUTATION IN VICIA FALA SEEDS IRRADIATED WITH LASER CO_2

本试验采用功率密度为394mW/cm^2的CO_2激光照射蚕豆萌动种子,观察其对蚕豆根尖细胞染色体畸变及幼苗生长的影响,探讨CO_2激光照射后蚕豆植株农艺性状的变异及其遗传传递情况.试验结果表明:CO_2激光不同时间照射蚕豆萌动种子,均能使根尖细胞产生染色体畸变,且畸变类型相似;同时,对L_1幼苗生长有明显的抑制作用,L_2植株高度、分枝数及结荚数等农艺性状均产生不同程度的变异.其中分枝数这一性状的变异能传递给L_3,其余性状的变异则属生理损伤所致.

In this experiment, sprouting seed of Vicia faba were irradiated with laser CO2 of 394 mW/cm2.Chromosomal aberration of root tip cells of Vicia faba and growing of seedlings were observed. Inquired is the agro-character of such mutation and inheritance in Vicia faba. Results show that laser CO2 can give rise to chromosomal aberration and induce similar chromsomal aberration in different groups. Laser CO2 prominently inhibits growth of seedlings L1 . Mutaion in L2 plant height, number of branches and rate of fuition etc.can be induced to varying degrees. The number of branches can transmit to L3, but plant height and fruition belong to physiological damage.