实热证、虚热证造模大鼠甲状腺超微结构和功能变化的联系

Relationship between Thyroid Ultrastructural and Functional Changes in Mice Pattern between Heat of Excess Type and Fever of Deficiency Type

目的 :观察“实热证”、“虚热证”造模大鼠血清甲状腺激素水平及甲状腺超微结构改变与机体能量代谢的关系。方法 :本实验运用放射免疫测定和电镜技术研究了实热证、虚热证模型大鼠血清中甲状腺的激素 (T3、T4 、rT3)和促甲状腺激素 (TSH)浓度的变化 ,并在电镜下观察了甲状腺的超微结构。结果 :虚热证造模大鼠血清中T3、T4 水平显著升高 ,rT3 水平呈下降趋势 ,TSH水平无明显变化 ;实热证造模大鼠血清中T3、T4 、rT3 和TSH浓度均无明显变化。在电镜下 ,虚热证造模大鼠甲状腺滤泡上皮细胞超微结构改变比实热证显著 ,与血清中激素浓度变化意义基本相同。结论 :热证大鼠甲状腺激素水平的变化与该组织上皮细胞超微结构的变化呈平行关系。

Objective:To observe the connexion of the levels of thyroxine and ultrastructure of the thyroid gland and energy metabolism change of functional.Method:The levels of thyroxine(T 3、T 4、rT 3)and thyroid stimulating hormone(TSH) in rat serum were measured by radioimmunoassay,and ultrastructure of the thyroid gland were examined by transmission electron microscopy(TEM)in mice pattern between heat of excess type and fever of deficiency type.Result:The results showed that the levels of T 3 and T 4 in serum significantly increased and the levels rT 3 of decreased,the level of TSH being unchanged obviously in fever of deficiency type;the levels of T 3、T 4、rT 3 and TSH did not increase obviously in heat of excess type.These evidences were almost in accord with the morphological changes of the thyroid follicular epithelial cell,in other words,the changes of ultrastructure of the thyroid follicular epithelial cell were more significant in fever of deficiency type than those in heat of excess type.Conclusion:The levels of thyroxine in mice pattern of Re syndrome are paralles with the changes of ultrastructure of the thyroid follicular epithelial cell.