SLE患者外周血单一核细胞凋亡率与病情活动性的相关性研究

Correlation between Apoptosis of Peripheral Blood Mononuclear Cells and Disease Activity in SLE Patients

目的研究ＳＬＥ患者外周血单一核细胞（ＰＢＭＣ）凋亡、自身抗体、病情活动性的相互关系。方法用吖啶橙染色法检测新鲜 ＰＢＭＣ凋亡率；免疫印迹法检测自身抗体；按文献方法计算 ＳＬＥ病情活动指数（ＳＬＥＤＡＩ）。结果ＳＬＥ患者ＰＢＭＣ凋亡率升高，并与ＳＬＥＤＡＩ高度正相关（Ｐ＜０．０１）；自身抗体种类的多少与ＰＢＭＣ凋亡率、ＳＬＥＤＡＩ均无明显相关性（Ｐ＞０．０５）。结论ＳＬＥ患者ＰＢＭＣ凋亡与其病情有关。

Objective To investigate the relationship between apoptosis of peripheral blood mononuclear cells(PBMC),au- toantibodies and disease activity in patients with systemic lupus erythematosus(SLE). Methods Apoptosis of PBMC and autoan- tibodies were examined by means of AO staining and immunoblotting resistively; disease activity was determined by SLE disease activity index(SLEDAI). Resuts The percentage of apoptosis of PBMC from SLE patients was higher than that of normal con- trols and significantly correlated with SLEDAI while the kinds of autoantibodies did not. Conclusion The apoptosis of PBMC correlates to the disease activity in SLE patients.