酒精对原代培养大鼠小脑细胞的损害

The damage of alcohol to cerebellar cells from primary cultured rats

原代培养的新生大鼠小脑颗粒神经元及小脑细胞经不同浓度的酒精进行处理 ,用倒置显微镜及四甲基偶氮唑蓝(MTT)法测定细胞存活率。结果酒精浓度在 5～ 40mmol/L时 ,细胞存活率呈剂量依赖性降低。酒精浓度增至 16 0mmol/L时 ,颗粒神经元的存活率回升。提示酒精对原代培养大鼠小脑细胞有细胞毒性作用。在高浓度 ( 16 0mmol/L)时 。

The cerebellar granule neurons and cerebellar cells from newborn primary cultured rats were treated with alcohol of different concentration. The cytotoxicity of alcohol on rats was observed. The survival rate of cells was detected by using an inverted microscope and MTT assay. Results showed that the survival rate of the rat cerebellar cells presented a dosage-dependent decrease when the concentration of the alcohol was from 5～40mmol/L, when the concentration reached 160mmol/L, the survival rate of rat cerebellar granule neurons rose again. This study suggests that alcohol has a cytotoxity to primary cultured rat cerebellar cells but at a high concentration its toxicity to cerebellar granule neurons of primary cultured rats is reduced.