刺梨汁对金黄地鼠的抗动脉粥样硬化作用

Effect of Rose Roxburghii Tratt Juice on Atherosclerosis in Hypercholesterolemic Hamsters

为寻找有效抗脂蛋白氧化剂防治动脉粥样硬化 ,在建立金黄地鼠的动脉粥样硬化模型过程中 ,分别补充维生素C、维生素E及刺梨汁 ,分析血浆脂质、抗氧化剂水平 ,低密度脂蛋白氧化易感性及动脉粥样硬化发生程度的变化。结果发现 ,高胆固醇饲料喂养 10周后 ,各组实验动物血脂水平均升高 ,且伴有主动脉粥样硬化斑块的形成 ;各抗氧化干预组血浆中相应的抗氧化剂水平均显著升高 ,低密度脂蛋白抗氧化性增强 (维生素C组、维生素E组、刺梨汁组和对照组的低密度脂蛋白氧化延迟时间分别为 2 2 1± 5 6min、2 2 2± 6 0min、2 48± 48min和 181± 47min ,组间比较P <0 .0 5 ) ,动脉粥样硬化斑块面积均显著减少 (维生素C组、维生素E组、刺梨汁组和对照组粥样硬化斑块程度分别为 2 .6 3%± 1.35 %、2 .44 %± 1.47%、1.43%± 0 .92 %和 5 .6 2 %± 1.2 8% ,组间比较P <0 .0 0 1) ,以刺梨汁组减少最明显。相关分析表明 ,主动脉粥样硬化面积程度与低密度脂蛋白氧化延迟时间呈显著负相关 (r =- 0 .42 ,P <0 .0 5 ) ,低密度脂蛋白氧化延迟时间与血浆维生素E水平呈正相关 (r=0 .36 ,P <0 .0 5 )。结果提示 ,抗氧化剂改善低密度脂蛋白氧化易感性 ,降低动脉粥样硬化的发生。

Aim To look for effective antioxidant to reduce risk of atherosclerosis. Methods The golden harmsters were fed for 10 weeks on a hypercholesterolemic diet. The animals received either vitamin C, vitamin E or juice of rose roxburghii tratt (JRRT) supplement in their diet except control animals. Results The level of corresponding antioxidants in various antioxidant-supplemented animals were increased compared to controls. The antioxidants induced the prolongation of lag time in low density lipoprotein (LDL) oxidation in vitamin C, vitamin E, JRRT groups compared with controls (221±56 min, 222±60 min, 248±48 min, and 181±47 min, compared between groups, P<0.05, respectively) and decreased the area of atherosclerosis lesion (2.63%±1.35%, 2.44%±1.47%, 1.43%±0.92%, and 5.62%±1.28%, compared between groups, P<0.001, respectively). Regression analysis showed that there was a negative relationship between the area of atherosclerosis lesion and LDL susceptibility. LDL susceptibilty was also correlated with the plasma level of vitamin E. Conclusions Antioxidants decrease extent of atherosclerosis lesion, and this may be caused by change in susceptibility of LDL oxidation. JRRT is a strong antioxidant for reducing risk of atherosclerosis.