染色体不稳定性及HPV、CMV感染与宫颈癌发病的关系

Correlation between chromosome instability,HPV CMV infection and the occurrence of cervical carcinoma.

目的 :探讨染色体数目畸变、脆性位点发生率、姐妹染色单体交换发生率、细胞内微核发生率、宫颈分泌物内人乳头状瘤病毒 (HPV)感染、巨细胞病毒 (CMV)感染与子宫颈癌的关系。方法 :对 30例子宫颈癌患者 ,做外周血细胞培养 ,分析染色体畸变、姐妹染色单体交换、脆点和微核。用PCR方法扩增宫颈分泌物HPV16 -DNA、HPV18-DNA、CMV -DNA。结果 :染色体畸变 ,姐妹染色单体交换发生率 ,脆性位点、细胞内微核发生率与正常对照组差异有显著性 ,宫颈癌组HPV16 -DNA、HPV18-DNA和CMV -DNA感染阳性率分别为 50 %、46 .67%和 53 .33 % ,3者均为阳性者占 2 0 %。结论 :染色体不稳定性增加及HPV。

Objective:To study chromosomal aberration,rate of chromosomal fragility,rate of sister chromatic exchange(SCE),rate of micronucleus in peripheral blood cell of patients with cervical carcinoma and DNA of human papillomavirus and cytomegalovirus.Methods:According to FIGO clinical diagnosis of cervical carcinoma in 1988,the authors collected 30 cervical carcinoma specimens and analysed their chromosomal aberration,chromosomal fragity,micronucleus in cell and a mplifed DNA of human papillomavirus and cytomegalovirus by polymerase chain reaction(PCR)from the patient's cervical secretion.Results:Significant difference of chromosomal aberration,chromosomal fragility,sister chromatic exchange were observed between the normal control and the cervical carcinoma groups.Positive rate of HPV 16 -DNA and HPV 18 -DVA and CMV-DNA were 50% and 46.67% and 53.33% .Respectively,significant higher than those of the control group's. Conclusions:The occurrence and development of cervical carcinoma is correlated with infection of HPV and CMV. The instability of chromosome of the patients is increased.