GnRH-a体外对人卵巢黄素化颗粒细胞雌二醇和孕酮分泌量的影响

Effect of gonadotropin-releasing hormone agonist on steroidogenesis of cultured human luteinized granulosa cells in vitro.

目的 :观察促性腺素释放激素激动剂 (GnRH -a)在体外对人卵巢黄素化颗粒细胞雌二醇 (E2 )和孕酮 (P)分泌量的影响。方法 :培养人卵巢黄素化颗粒细胞 ,分别用终浓度为 1.0、10 .0、10 0 .0ng ml的GnRH -a刺激 ,同时设对照组 ,培养时间为 2、4、6d。用放射免疫法检测黄素化颗粒细胞E2 和P的分泌量。结果 :培养 2d ,GnRH -a中、高浓度组E2 和P分泌量分别为 (12 2± 37) %、(12 8± 2 4 ) % ;(143± 32 ) %、(137± 2 9) %对照组为10 0 % ,均显著高于对照组 (P <0 .0 5) ;低浓度组E2 和P分泌量与对照组差异无显著性。随着细胞培养天数的增加 ,GnRH -a中、高浓度组E2 分泌量明显低于对照组 ,高浓度组E2 分泌量与培养天数呈负相关 (r =- 0 .75,P <0 .0 5)。结论 :GnRH

Objective: To observe the effect of gonadotropin-releasing hormone agonist(GnRH-a) on steroidogenesis of cultured human luteinized granulosa cells in vitro.Methods: Human luteinized granulosa cells were cultured in serum-free McCoy'5a medium.After stimulating with various concentrations of GnRH-a for 2, 4 and 6 days.Estradoil (E 2) and progesterone (P) levels in the media were measured by radioimmunoassay.Results: When stimulated with different concentrations (10.0ng/ml and 100.0ng/ml)of GnRH-a for 2 days, E 2 and P levels produced by luteinized granulosa cells increased and were significantly higher than those of control (P<0.05).In lower concentration group there were no significantly difference of E 2 and P levels when compared with control group.After stimulated with different concentration of GnRH-a for 4 and 6 days,the E 2 levels in media significantly decreased except for the low concentration group.There was a significant positive correlation between the E 2 level and days under the stimulation of high concentration GnRH-a(r=-0.75,P<0.05).Conclusion:The effects of GnRH-a on luteinized granulosa cells lay on both the concentration and time.