LT受体拮抗剂对大鼠肺纤维化LTB_4及血F_n的影响

The Effect of LT-receptor Antagonist to LTB_4 and F_n in Pulmonary Fibrosis

45只大鼠随机分为 3组 ,对照组、模型组、治疗组各 15只。治疗组气管内单次灌注博莱霉素 (5 mg)后每日以安可来 (ACL,10 mg/ kg)灌胃 ;模型组气管内灌注同治疗组 ,灌胃以生理盐水代替。 3组动物在气管内灌注后分别于第 7、14、2 8d随机处死 5只 ,取左肺下叶 1g匀浆测 L TB4,心腔血测纤维联结蛋白 (Fn)。模型组和治疗组在气管内灌注后白三烯 B4(L TB4)迅速上升 ,第 7d达高峰 ,第 2 8d模型组 L TB4仍较高 ,治疗组 L TB4明显下降 ;Fn浓度模型组在第 7d明显升高 ,治疗组及对照组较低。结论 :在鼠实验性肺间质化过程中 L TB4明显增高 。

Forty five rats were divided into three groups in random control group, untreated model group, ACL treated group. On experimental Day(Day O),the rats were intratracheally instilled Bleomycin (5 mg/kg)or sterile saline, and then treated with ACL(10mg/kg)or saline until they were killed. At the date of 7th,14th,28th after instillation,fifteen rats of each group were sacrificed, the 1.0 gram lower lobe of left lungs were harvested and jellied for determination of LTB 4,the blood was collected for determination of Fn. After BLM instillation,LTB 4 of treated group and model group was elevated quickly and peaked at 7th day, then treated group declined notably but model group slowly. Fn of model group was much more elevated than that of other group's, Conclusions: LTB 4 acts on an important role in the development of pulmonary fibrosis