人生长激素对人结肠癌细胞LS-174-T增殖周期的影响

Effect of human growth hormone on cell cycle kinetics in colonic cancer cells

目的 :研究人生长激素 (h GH)对体外培养的结肠癌细胞株增殖周期和 DNA倍体的影响和意义。 方法 :取指数生长期的结肠癌细胞株 L S- 174- T(简称 174) ,以顺铂和 (或 )不同浓度的 h GH体外培养 ,2 4h后以流式细胞仪测定细胞增殖周期等指标。 结果 :h GH能诱导 174细胞 S期数率显著上升 (P<0 .0 1) ,G2 / M期参数下调 (P<0 .0 1) ,DNA指数未见增高。加用顺铂后细胞大量凋亡 ,细胞存活率显著下降 (P<0 .0 1) ,各组 S期百分数或显著下降 ,或与对照组无差异。 结论 :h GH体外作用于 174细胞 ,能促使细胞进入对化疗药敏感的 DNA合成期 ,没有上调 DNA倍体数 ;顺铂能诱导细胞大量凋亡 ,并且抑制 h

Objectives:The effects of human growth hormone (hGH) on cell cycle kinetics were studied in human colonic cancer cell line, LS 174 T cell. Methods: The cells were cultured in RPMI-1640 medium for 24 hours with hGH at concerntration of 50, 100, 200 ng/ml with or without cisplatin. Cell cycle kinetics was analyzed by flow cytometry. Results: The significant effects of hGH on cell cycle kinetics were found in the cell line. Percentage of S phase increase ( P <0.01) and percentage of G 2/M phase decreased ( P <0.01).DNA Index (DI) didn't increase.The cells had a lot of apoptosis and percentage of S phase decreased ( P <0.01) with addition of cisplatin. Conclusions: In vitro hGH may induce the proliferation of colonic cancer cells. Cisplatin may suppress the stimulatory effects of hGH on S phase and increase the apoptosis of colonic cancer cells.