谷氨酰胺对短肠综合征大鼠残留小肠、结肠形态的影响

The effect of Glutamine on the shape of residual intestine and colon in rats with short bowel syndrome

目的 :观察谷氨酰胺 (Gln)对短肠综合征大鼠残留小肠、结肠形态的影响。 方法 :2 3只雄性 SD大鼠切除80 %小肠 ,随机分为三组 :饮食组 (n=8)大鼠术后自由进食 ;全胃肠外营养 (TPN)组 (n=8)输 TPN标准液 ;Gln组(n=7)输 TPN + Gln液 ;正常大鼠 8只 ,作为正常对照组。术后第 7天 ,称体重 ,取残留空肠、回肠、结肠进行组织学检查 (包括光镜和电镜 )。 结果 :饮食组和 Gln组术前体重无明显差异 ,但术后体重有明显差异 ;饮食组空肠粘膜绒毛高度 (VH )和粘膜厚度 (MT)、回肠粘膜的 VH均明显大于正常组 ;TPN组空肠粘膜 VH、MT明显小于正常组 ;回肠粘膜隐窝深度 (CD)、MT亦明显小于正常组 ;Gln组空肠和回肠粘膜 VH、CD和 MT明显大于 TPN组 ;饮食组结肠 MT明显大于正常组 ,Gln组结肠 MT明显大于 TPN组。 结论 :80 %小肠切除后 ,残留小肠发生代偿性改变 ,食物刺激是残留小肠代偿的重要因素 ;但这种代偿不完全 ,TPN可维持机体体重 ,但可引起残留小肠粘膜萎缩 ;Gln能阻止 TPN引起残留小肠粘膜萎缩 ,促进残留小肠代偿 ;同时

Objectives:To investigate the effect of glutamine on the shape of residual intestine and colon in rats with short bowel syndrome. Methods:23 male Sprague Dawley rats,underwent a 80% small bowel resection,were randomly divided into three groups:food group( n =8) rats,fed rat chow and water libitum after operation;TPN group( n =8),infused with Gln supplied TPN;and normal control group.On seventh day after operation,rats were weighted and remaining jejunum、remaining ileum and colon were harvested for histological observation(light microscopy and electron microscopy). Results:There was significant difference in rat average weight between food group and Gln group after operation.Jejunal mucosal villus height(VH) and mucosal thickness(MT) and ileal mucosal VH in food group were significantly increased than those in control group.Jejunal mucosal VH and MT in control group were significantly higher than in TPN group.Ileal mucosal crypt depth(CD) and MT in control group were also significantly higher than in TPN group.Jejunal and ileal mucosal VH、CD and MT in Gln group were significantly higher than in TPN group.Colonic MT in food group was significantly higher than in control group.Colonic MT in Gln group was significantly bigger than in TPN group. Conclusions:After 80% intestinal resection,the remaining intestine can develop the adaptation,but the adaptation is incomplete.TPN therapy can maintain body weight,but only TPN can not result in the adaption.Gln supplied TPN can stop the remaining itestinal mucosal atrophy,and promote the remaining intestinal adaptation and colonic mucosal hypertrophy.