摘要
用酶解法观察了枳和宜昌橙根尖细胞的染色体。根尖经固定后于37℃下在含有6％果胶酶和2％纤维索酶的溶液中解离1.5～2小时,用DAPI或吉姆萨染色均能获得处于分裂晚前期或中期的清晰的染色体。
The chromosomes in root tip cells of Trifoliate orange(Poncirus trifoliata Raf. )and Ichang papeda(Citrus ichangensis Swingle)were observed by enzymic maceration technique. After fixation, the root tips were macerated in a solution containing 6％ pectinase and 5％ cellulase for 1.5～2 hours at 37℃. After stained with DAPI or Giemsa, the chromosomes at late-prophase or metaphase in mitosis could elearty be seen.
出处
《中国柑桔》
1992年第1期10-12,共3页
South China Fruits
关键词
柑桔
染色体
酶解法
Enzymic maceration
Chromosome observation
