摘要
为了检测胸腺素 β4的表达水平与细胞生长状态的关系 ,选用胸腺素 β4特异性的引物 ,运用RT PCR的方法 ,分别检测了不同增殖状态的小鼠骨髓内皮细胞和HL 60细胞以及外周血单个核细胞中胸腺素 β4的表达情况。结果显示 ,当内皮细胞处于活跃增殖时 ,它们的胸腺素 β4表达水平高 ,否则表达水平低。HL 60细胞中胸腺素 β4表达水平较人外周血单个核细胞中的高。
To investigate the relationship between the growth situation of the cells and the expression level of thymosin β4, the specific primer of thymosin β4 was selected to test the expression of thymosin-β gene in murine bone marrow endothelial cells in different proliferation states and in HL-60 cells and peripheral blood mononuclear cells by RT-PCR. The results showed that the expression level of thymosin β4 in the murine bone marrow endothelial cells with active proliferation was high, otherwise it was low. The thymosin β4 gene expression level was higher in HL-60 cells than that in human peripheral blood mononuclear cells. It was suggested that the expression level of thymosin β4 mRNA is closely related to cell growth.
出处
《中国实验血液学杂志》
CAS
CSCD
2001年第1期10-13,共4页
Journal of Experimental Hematology
基金
国家自然科学基金资助项目 编号 3 9970 0 92~~