白血病病人与健康人白细胞氧自由基及耗氧量的比较

Comparing the Oxygen Free Radicals and Oxygen Consumption of Leukocytes in Leukemia Patients and Healthy Persons

利用电子自旋共振 (ESR)自旋捕集与探针技术 ,观察白血病病人与健康人白细胞氧代谢过程中释放活性氧自由基的种类及其耗氧量的变化。结果发现 ,白血病病人白细胞经豆蔻酰佛波醇乙酯 (PMA)刺激只捕集到微弱的羟自由基 (·OH)信号 ,刺激前后耗氧量差别不显著 ;健康人白细胞可得到超氧阴离子自由基 (O 2 )的ESR波谱信号 ,耗氧量较正常呼吸即未受PMA刺激时大大增加 (P <0 .0 0 1)。两组白细胞耗氧量比较 ,未受刺激时两者无明显差别 ;刺激后 ,健康组明显高于白血病组 (P <0 .0 0 1)。结论说明白血病病人白细胞缺乏呼吸爆发功能 。

Using the electron spin resonance(ESR) and probe technique, the species of reactive oxygen free radicals and the oxygen consumption were observed in the metabolic process of oxygen of leukocytes from leukemia patients and healthy persons. Results showed that weak ESR spectrum of hydroxyl radical ( ·OH) signal was detected in the leukemia patient′s leukocytes, there was no significant difference in oxygen consumption with or without PMA stimulation; superoxide anion(O XZ(180 2 ) in normal leukocytes was detected, and oxygen consumption increased markedly compared to the normal respiration without PMA stimulation(P<0.001). There was no significant difference in oxygen consumption between leukemic and normal leukocytes without PMA stimulation; however, it was higher in normal leukocyte than in leukemic leukoeytes after PMA stimulation (P< 0.001). These observations suggest that dysfunction of respiratory burst is existed in leukemic leukocytes, and their oxygen consumption is markedly lower than that of normal leukocytes.