一株能支持造血的人脐静脉内皮细胞系的建立

Establishment of a Human Umbilical Vein Endothelial Cell Line Supporting Hematopoiesis In Vitro

为了方便研究人脐静脉内皮细胞在造血调控和心血管系统中的作用 ,利用脂质体介导基因转染的方法 ,将SV4 0大T抗原导入原代人脐静脉内皮细胞 ,建立了至少可传代培养 2 5代的人脐静脉内皮细胞系IEC。免疫细胞化学染色显示IECⅧ因子阳性。流式细胞术检测I型荆豆凝集素结合率为 97%。染色体分析表明IEC细胞内染色体结构和数目发生了改变。体外实验表明 。

Endothelial cells are the critical cell component of hematopoietic microenvironment. For the purpose of facilitating the study on modulating effect of endothelial cells in hematopoiesis, a human umbilical vein cell line, IEC, was established. Primary human umbilical vein endothelial cells were transfected with the plasmid pSV 3neo carried SV40 large T antigen by lipofection. The IEC cells expressed factor Ⅷ and the UEA I-binding ratio was about 97%. The chromosome variation was existed in the cell line, with the karyotype 45, XX, -18, 18q +. The cell line retained the proliferative capacity at least 25 passages. IEC cells stimulated the growth of granulocyte-macrophage progenitor cells in coculture of cord blood CD34 + cells with IEC cells. It is concluded that IEC cell line possessed the biological features of endothelial cell and supported hematopoiesis in vitro.