多种细胞因子激活骨髓免疫细胞及细胞毒作用

Activation and Cytotoxicity of Bone Marrow Immunocytes by Using Various Cytokines

探讨不同细胞因子组合激活骨髓中免疫细胞数量、提高细胞毒作用和保留造血祖细胞的情况。将骨髓细胞分为 4组进行培养 :①对照组 :不加任何细胞因子 ;②IL 2组 :用IL 2激活骨髓细胞 ;③CD3 AK组 :用IL 2和CD3 McAb激活骨髓细胞 ;④CIK组 :加入IFN γ ,IL 1,IL 2和CD3 McAb激活骨髓细胞。培养前后各组取单个核细胞进行CFU GM和CD3表型检测 ;培养过程中观察各组细胞数量变化 ;用MTT法检测各组培养后的细胞毒作用。结果发现 ,CD3 AK和CIK组培养后CD3+细胞比例明显增加 ,且两组的细胞总数增加和杀瘤活性明显高于对照组和IL 2组 (P <0 .0 5 ) ;各组培养后CFU GM均有所减少 ,但各组间无显著差别。本试验表明 ,IL 2和CD3 McAb组合既能刺激骨髓免疫细胞增殖并提高细胞毒作用 ,又能在培养后保留足够的造血干 祖细胞 。

The study aimed to explore the changes of the immunocyte quantities and cytotoxicity in bone marrow, and how many hematopoietic progenitor cells retained after the bone marrow cells were activated by different combinations of various cytokines. Bone marrow cells were divided into four groups and were cultured in vitro: ① Control: no cytokines were added. ② IL-2 group: bone marrow cells were activated by IL-2. ③ CD3-AK group: bone marrow cells were activated by IL-2 and CD3-McAb. ④ CIK group: IFN-γ, IL-1, IL-2 and CD3-McAb were added. CFU-GM assay and CD3 phenotype detection were performed before and after activating culture in all groups. The changes of cell quantities during culture and cytotoxicity of cultured cells were tested. CD3 positive cells markedly increased in both CD3-AK and CIK groups. The cell numbers and cytotoxicity of CD3-AK and CIK groups were higher than those of control or IL-2 group obviously after culture(P<0.05). CFU-GM were decreased in all groups after culture and there had no significant difference among four groups. The combination of IL-2 and CD3-McAb not only stimulates the proliferation of marrow immunocytes and increases their cytotoxicity but retains enough hematopoietic progenitor cells as well. This combination of cytokines can be used to purge autologous bone marrow in vitro.