摘要
To elucidate the role of fetal bone marrow stromal cells(FBMSC) in cooperation with exogenous cytokines in supporting the in vitro expansion of cord blood CD34 + cells which were purified by negative immunomagnetic selection, FBMSCs were cultured with different combinations of cytokines including SCF, IL-3, IL-6, FL, G-CSF and EPO in a 14-day liquid culture system. The results showed FBMSC plus SCF, IL-3, IL-6, FL and EPO was the most effective combination which increased total nucleated cells, CFU-GM, BFU-E and CD34 + cells by (692.4±52.7) fold, (237.1±106.6) fold, (114.8±32.8) fold and (25.3±10.1) fold, respectively. Our studies indicated that fetal bone marrow stromal cells combined with above-mentioned cytokines can efficiently expand cord blood CD34 + cells.
To elucidate the role of fetal bone marrow stromal cells(FBMSC) in cooperation with exogenous cytokines in supporting the in vitro expansion of cord blood CD34 + cells which were purified by negative immunomagnetic selection, FBMSCs were cultured with different combinations of cytokines including SCF, IL-3, IL-6, FL, G-CSF and EPO in a 14-day liquid culture system. The results showed FBMSC plus SCF, IL-3, IL-6, FL and EPO was the most effective combination which increased total nucleated cells, CFU-GM, BFU-E and CD34 + cells by (692.4±52.7) fold, (237.1±106.6) fold, (114.8±32.8) fold and (25.3±10.1) fold, respectively. Our studies indicated that fetal bone marrow stromal cells combined with above-mentioned cytokines can efficiently expand cord blood CD34 + cells.
出处
《中国实验血液学杂志》
CAS
CSCD
2001年第1期93-94,共2页
Journal of Experimental Hematology
基金
国家自然科学基金资助 编号 3 980 0 0 5 5
