钙及高压氧对卵巢切除大鼠骨丢失影响的实验研究

Effect of calcium and hyperbaric oxygenation on bone loss in ovariectomized rats

目的 评价纳米碳酸钙及高压氧对雌激素缺乏引起的骨丢失的预防作用。方法 采用 3月龄Wistar雌性大鼠切除双侧卵巢并用低钙饲料喂养作动物模型 ,术后分别给予钙剂及高压氧 ,连续 14周。结果 与假手术组 (Sham)相比去势组 (OVX)骨密度、股骨生物力学性质、生化、骨形态计量指标均有显著差异 ;钙剂组 (OVX +Ca)及高压氧组 (OVX +HBO)与OVX组比较 ,股骨干及股骨近段骨密度、股骨最大载荷、弹性桡度、最大应力、弹性模量显著升高 ,血清TRAP、ALP显著下降 ,高压氧组股骨弹性载荷、弹性应力、弹性应变的升高及血清TRAP、ALP的下降均较钙剂组明显。结论 纳米碳酸钙及高压氧能在一定程度上抑制去势低钙饲料喂养的Wistar大鼠骨密度及骨生物力学性能下降。

Objective\ To evaluate the preventive effect of nanometer calcium carbonate and hyperbaric oxygenation(HBO) on bone loss inducd by depletion of estrogen. Methods\ Bone mineral density(BMD),biomechanical properties and biochemical histomorphological parameters were investigated in 3\|month\|old ovariectomized Wistar rats on a low\|calcium diet.Administration of calcium and HBO was started immediately after ovariectomy and continued for 14 weeks. Results\ BMD,biomechanical properties, biochemical and histomorphological parameters significantly changed in OVX rats,compared with sham\|operated rats.Significant increase was found in OVX+Ca and HBO rats,compared with OVX rats in regard to the BMD values of proximal femoral segment and femoral diaphysis,and in regard to the biomechanical parameters of femur such as maximum load,elastic deformation,maximum stress and elastic modulus.With respect to the change in biochemical parameters,the OVX+Ca rats and OVX+HBO rats showed a significant decrement in serum TRAP and ALP,compared with OVX group.The increment of elastic load,elastic stress,elastic strain and the decrement of serum TRAP and ALP were more obvious in OVX+HBO group than in OVX+Ca group. Conclusion\ Nanometer calcium carbonate supplementation and hyperbaric oxygenation can inhibit bone loss and the decrease of biomechanical properties in ovariectomized Wistar rats on a low calcium diet.