肺高压肺血管重建大鼠基质金属蛋白酶1基因表达

Gene Expression of MMP-1 of Pulmonary Vascular Remodeling in Rats with Pulmonary Hypertension

目的 探讨基质金属蛋白酶 1(MMP 1)基因表达与肺高压形成的关系。方法 利用野百合碱(monocrotalineMCT)诱导的大鼠肺高压动物模型 ,经导管介入测定大鼠肺动脉平均压力 ,逆转录定量聚合酶链反应 (RT PCR)检测不同时间点大鼠肺组织MMP 1mRNA相对表达水平。结果 实验第 3周肺动脉压力已明显升高 ,以第 4周为最高 ,而大鼠肺组织MMP 1mRNA表达水平以实验第 2周最为明显 ,第 3,4周表达水平下调 ,但仍高于第 1周。结论 MMP 1早期基因表达升高参与细胞外基质屏障的降解 ,对肺血管重建可能起了触发作用 ,实验后期表达水平下调可能是导致细胞外基质积聚的重要原因之一。

Objective To explore the etiology of pulmonary hypertension with relation to the pulmonary expression of matrix metalloprotease1 (MMP 1). Methods The pulmonary hypertension animal models were induced by monocrotaline (MCT), the pulmonary arterial pressure was measured by cathetrization, and the pulmonary MMP 1 mRNA expression was studied by RT PCR. Results The pulmonary arterial pressure increased significantly in the 3rd week after the induction of MCT and reached the peak in the 4th weak; the pulmonary MMP 1 mRNA expression increased significantly in the 2nd week and then decreased greatly in the 3rd week, and still it was higher than that in the 1st weak. Conclusions Changed MMP 1 expression might play a part in the development of pulmonary hypertension. MMP 1 overexpression might play the role of target during the earlier stage of pulmonary arterial remodeling.