被动吸烟大鼠肺泡灌洗液炎性细胞及呼吸道病理形态学观察

Observation of Inflammatory Cells in Bronchoalveolar Lavage Fluid and Pathological Morphology of the Airway in Passive Smoking Rats

为观察被动吸烟大鼠肺泡灌洗液炎性细胞及呼吸道病理形态学改变 ,将清洁级 SD大鼠随机分成两组 ,吸烟组 ( n=1 3 )置于隔离器内被动吸烟 2 h/ d,共 2 0 d,对照组置隔离器内 2 h/ d,无被动吸烟。 2 0 d后行支气管肺泡灌洗液 ( BAL F)炎性细胞计数及分类 ,并作右总支气管气道表面电镜下的病理形态学观察及肺组织学检查。结果显示吸烟组大鼠BAL F中炎性细胞计数为 ( 46.1± 2 8.3 )× 1 0 6 / L,较对照组 ( 1 7.7± 9.9)× 1 0 6 / L 明显增高 ( P<0 .0 5)。光镜检查示吸烟组小气道和肺组织炎性细胞浸润 ,扫描电镜观察见柱状上皮细胞纤毛倒伏凌乱 ,部分脱落 ;对照组未见明显异常。烟雾刺激导致大鼠呼吸道炎性细胞增多 ,炎症反应增强及纤毛功能减退 。

Female clean SD rats were divided into two groups. Group Ⅰ,13 rats were layed in the isolator for passive smoking for two hours per day. Group Ⅱ, as control group, 10 rats were layed in the isolator without passive smoking for two hours per day. Twenty days later, bronchoalveolar lavage fluid (BALF) were collected for counting and sorting the inflammatory cells; the surface of the main right bronchus was observed by scanning electron microscope; lung tissues were observed by light microscope. The results showed that the number of total cells in BALF in Group Ⅰ[(46.1±28.3)×10 6/L] was higher than that in Group Ⅱ[(17.7±9.9)×10 6/L] ( P <0.05).The inflammatory cells were infiltrated in the wall of small airways and lung tissues; the fimbriae of columnar epithelial cells of airway were falled and in disorder, some of them shed. The results suggested that the stimulation of smoke induced increase of inflammatory reaction of the airway and the function of fimbriae decreased, these may lead to the injury of the airway.