小鼠胚胎库的现状及胚胎和配合子冷冻保存的应用前景

The Trend of Mouse Embryos/Spermatozoa Bank and Cryopreservation of Mouse Embryos and Gametes

近年来 ,逐渐增多的小鼠突变系资源已对现有的动物设施和品种 (系 )的保存方法提出严峻的挑战。小鼠胚胎和配合子的低温保存是目前保存小鼠种群资源中应用最为广泛的方法 ,通过低温保存不仅使原有的或新开发的小鼠品系得以安全的、长期的保存下来 ,同时也使这些小鼠模型的规模化生产、推广普及变得更为简单化。无疑 ,小鼠模型的保存将为 2

Mouse genetics is setto play a pivotal role in the key post-genome challenge, specially in the study of human gene function.The normal mouse are mutated with the methods of gene engineering( such as transgenic,knock-out and knock-in) ,to promul- gate the human gene function.Butthe expanding mouse mutantresource thatwill result threatens to overwhelm the currently available animal facility space.As more and more genes are sequenced and cloned,the numberof new knock-out,knock-in and transgenic mice will undoubtedly increase. The bestand mostsafe method issimplified of extensive frozen archives now.Comprehensive mouse mutantarchives will provide an essential re- source for mammalian genetics throughout the2 1 st century.