rRNA技术及其在分子微生物生态上的应用

rRNA TECHNIQUE AND ITS APPLICATION IN MOLECULAR MICROBIAL ECOLOGY

传统的基于微生物培养与纯种分离的技术所具有的局限性 ,以及分子生物学及其有关技术的长足进展 ,使微生物生态学的研究进入了分子的阶段。其中rRNA技术的建立、发展及其成功应用 ,为分子微生物生态和微生物系统分类学的研究掀开了崭新的一页。对rRNA分子技术的研究进展、以之为基础的主要方法及其在环境微生物研究中的应用 。

Due to limitation of traditional techniques that based on microbial cultivation and pure culture isolation, great advance in molecular biology and relevant technologies have made microbial ecology enter a molecular stage. The establishment of rRNA techniques and their application has opened a new page for molecular microbial ecology and systematics. Major techniques and progresses of rRNA techniques and their application in microbial ecology are reviewed in detail. Potential problems during application and possible resolutions are discussed.