沙棘组织培养技术的研究

Studies of tissue culture techniques of Hippophae L.

本试验选用中国沙棘的种子和休眠枝条作为材料 ,研究沙棘不同外植体的离体培养技术。试验结果表明 ,沙棘的无菌苗子叶与休眠枝条上的休眠芽都可以作为离体培养的良好外植体。沙棘的最适分化培养基是 :1 /4MS+6- BA0 .30 mg/L+NAA0 .0 0 2 mg/L;增殖、壮芽培养基是 :1 /4MS+6- BA0 .1 mg/L+NAA0 .0 0 4 mg/L;最适生根培养基是 :1 /4MS+NAA 0 .0 5mg/L +IBA 0 .2 mg/L;愈伤组织诱导培养基是 :1 /4MS+2 ,4- D 0 .3mg/L。

The sterile plants and meristem of germinated dormancy buds of Hippophae rhamnoides L.were taken as explants cultured \%in vitro\% to induce the plant regeneration.The basic culture medium is 1/4 MS medium.The results showed that the shoot differentiation medium is 1/4 MS+6 BA 0.30 mg/L+NAA 0.002 mg/L,the multiplication medium is 1/4 MS+6 BA 0.1 mg/L+NAA 0.004 mg/L,The medium for rooting is 1/4 MS+NAA 0.05 mg/L+IBA 0.2 mg/L and the callus inducing medium is 1/4 MS+2,4 D 0.3 mg/L.