哮喘豚鼠外周血淋巴细胞凋亡及白细胞介素-5和粒细胞/巨噬细胞集落刺激因子mRNA表达的研究

Apoptosis and expression of mRNA Encoding IL－ 5 and GM－ CSF of peripheral lymphatic cell in asthmatic guinea pig

目的探讨哮喘豚鼠外周血淋巴细胞凋亡及白介素－ 5(IL－ 5)、粒细胞 /巨噬细胞集落刺激因子 (GM－ CSF)mRNA的表达。方法实验分为哮喘组和对照组，各 15只豚鼠。采用原位末端标记 (TUNEL)法检测豚鼠外周血淋巴细胞 IL－ 5、 GM－ CSFmRNA的表达。结果哮喘组外周血淋巴细胞凋亡率为 (3.50± 1.00)％，对照组为 (6.80± 1.50)％，哮喘组显著低于对照组 (P< 0.01)。哮喘组 IL－ 5、 CM－ CSFmRNA表达阳性率分别为 (58.00± 6.00)％和 (49.00± 4.00)％，对照组分别为 (30.00± 2.00)％和 (24.00± 2.00)％，哮喘组显著高于对照组。结论哮喘豚鼠外周血淋巴细胞 IL－ 5、 GM－ CSFmRNA的高表达，可能与淋巴细胞被激活、凋亡受抑制，从而分泌细胞因子增加有关。

Objective Toinvestigate apoptosis and expression of mRNA encoding IL－ 5 and GM－ CSF of peripheral lymphatic cell of asthmatic guinea pig.Method 30 guinea pigs were equally aasigned to asthmatic or control group. tumel was used to determine the rate of apoptosis and in situ hybridization(ISH)was used to examine the expression of mRNA encoking IL－ 5 and GM－ CSF of perpheral lymphatic cell. Result the rate of apoptosis in asthmatic group(3.50± 1.00％ )was much lower than that in control group (6.80± 1.50％ )(p<0.01).the positive rates of expression of mena encoding IL－ 5 and GM－ CSF in asthmatic group(58.00± 6.00％ ,49.00± 4.00％ )were much higher than that in control group(30.00± 2.00％ ,24.00± 2.00％ ).Conclusion The hyper－ expression of mRNA encoding IL－ 5 and GM－ CSF in perpheral lymphatic cell may be related with the activation as well as the decrease of apoptosis of lymphatic cell,which induced more secretion of cytokine.