高效液相色谱法分离测定大鼠肝线粒体膜磷脂

Determination of phospholipid in the mitochondial membrane of rat liver with HPLC

建立了HPLC方法、运用梯度洗脱同时分离测定大鼠肝线粒体膜中的心磷脂 (C)、磷脂酰肌醇 (PI)磷脂酰乙醇胺 (PE)、磷脂酰胆硷 (PC)、鞘磷脂 (SM)。结果表明 ,方法的线性关系、精密度、重现性较好。

A method was developed for the determination of cardiolipin,phosphatidyllinositol,phosphatidylserine,phosphatidylethanolamine,phosphatidylcholine and sphingomyelin in the mitochondrial membrane of rat liver with HPLC.The seperation was performed by gradient elution. The result indicated that the method was satisfying in its linear relationship, reproducibility and stability.