明胶纤维网粘附性及生物吸收性的实验研究

Experimental study on adhesiveness and biological absorption of gelatin fleece

目的 通过对明胶纤维网在出血创面粘附性能及生物吸收性能的研究,为临床应用和实验研究提供依据。方法 在兔背部取中厚皮片,造成均匀的出血创面,将明胶纤维网（GF）、氧化纤维素（OC）和明胶海绵（GL）三种局部止血材料随机贴敷于渗血创面,4种压力分别压30秒后,用张力换能器垂直向上牵引,二道生理记录仪描记粘附力（g/cm2）;将GF15mg埋入兔肝右叶后,分别于24小时、3天和 1,3,4,8周在埋药部位取标本,观察组织吸收及局部反应。结果 GF和OC与创面的粘附力显著大于GL（P<0.01）,GF随压力的增加粘附力逐渐增大,与OC,GL比较有显著差异（P<0.01）,GF对组织的反应较轻,8周时即可大部分吸收。结论 GF,OC与创面粘附力大于GL,增大压力可以增强GF的粘附力,证明GF是一种止血效果好、组织反应轻、可吸收的止血材料。

Objective To investigate the adhesiveness and absorption of gelatin fleece on the traumatic surface so as to provide references for its potentially clinical application. Methods A split skin graft of 0. 3 mm in depth was taken from each of 6 rabbits dorsal. Bleeding was treated with pressing one of the three types of hemostatic agents, Gelatin fleece (GF),Oxidized cellulose(OC) and Gelfoam(GL). After four different pressures were applied to each agent for 30 seconds, the agent was drawed up vertically through a tonotransductor and adhesiveness was recorded during this process. 15 mg GF were placed on hepatic trauma of rabbits. Then tissue absorption and local reaction were observed respectively after 24 hours, 3 days, and in 1, 3, 4 and 8 weeks afterwards. Results There were significant differences between the abhesive-ness of GF and OC to the bleeding surface, and GL(P<0.01). Moreover, the adhesiveness of GF to bleeding surface increased with pressing force, but the adhesiveness did not increase in OC and GL groups. GF was absorbed almost com-pltetely after 8 weeks with little histological reaction. Conclusions The adhesiveness of GF or OC to bleeding surface was greater than that of GL. Increase of pressing force might enhance the GF adhesiveness to bleeding surface. It was shown that GF was an absorbable material with better hemostatic effects and little tissue reaction.