美国白蛾核型多角体病毒超氧化物歧化酶基因的序列分析和表达

ANALYSIS AND EXPRESSION OF HYPHANTRIA CUNEA NUCLEAR POLYHEDROSIS VIRUS sod GENE

根据测序结果 ,HcNPVsod的核苷酸序列与BmNPVsod的完全一致 ,与AcNPVsod的核苷酸序列相比 ,同源性达到 97 2 % ;推测HcNPVsod编码 1 51个氨基酸 ,与BmNPVsod的完全一致 ,与AcNPVsod编码的氨基酸相比 ,有三个氨基酸的差别。按基酸序列分析表明 ,HcNPVSOD蛋白中含有对SOD结构和活性必需的氨基酸残基 ,在HcNPVsod中均是保守的。SOD活性测定表明酶活为 1 47 0

The sequencing results indicated that \%Hyphantria cunea\% nuclear polyhedrosis virus (HcNPV) \%sod\% gene open reading frame of 456 nt encoding protein of 151 amino acid,was identified to that of Bombyx mori nuclear polyhedrosis virus (BmNPV), and exhibited 97 2% homology at nucletde level to that of \%Autographa californica\% nuclear polyhedrosis virus (AcNPV),three amino acid residues difference in amino acid level with AcNPV sod.The essential amino acid residues for the construction and active could be detected in HcNPV sod.Activity of the SOD is 147 09U per milliliter E.coli.