摘要
利用SSR标记技术对大豆属11个种37份材料的遗传多样性进行分析,不同位点在种间的等位基因数为6~29,平均每个位点15.9个等位基因,Soja亚属的等位基因数是Glycine亚属的71.5%,并且Glycine亚属种间指纹图谱的差异大于Soja亚属种间的指纹图谱。SSR等位基因的主成分分析结果表明,大豆属中的Glycine亚属和Soja亚属的分类界限是比较明确的,利用第一主成分和第二主成分可较明显地区分开Glycine亚属和Soja亚属。通过UPGMA方法构建了大豆属11个种的遗传进化关系,Soja亚属中G.max、G. soja和G. gracilis3个种在系统分化树上界限是比较明显的,由此看来这3个种是独立存在的。
The genetic diversity among 11 species of genus Glycine (altogether 37 ac cessions) was evaluated through SSR analysis. The number of alleles in different lo ci ranges from 6 to 29, averaging 15.9 per locus; alleles of subgenus soja account for 71.5% of those in subgenus Glycine, and the fingerprinting among subgenus Glyci ne is more divergent than that of subgenus soja. Principal factor analysis shows th at the first and the second principal factor can classify genus Glycine into two gr oups which represent subgenus Glycine and subgenus soja. The results of UPGMA indic ate that G. mas, G. soja and G. gracilis in subgenus soja are different species.
基金
国家重点基础研究项目!(No.G1998010208)