应用(Dig)-DNA探针进行恶性疟流行病学调查

Use of(Dig)-DNA Probe for the Epidemiological Survey of Plasmodium Falciparum Malaria

重组的恶性疟原虫DNA片段用洋地黄毒苷配基标记后作为探针(pPF_(14)-F-Dig),以斑点杂交试验对海南省恶性疟流行区人群的274份血样进行检测,并同时作厚薄血片镜检。结果显示,274例样本中血片镜检阳性1例,带虫率为0.36%;pPF_(14)-F-Dig 探针检出阳性15例(其中1例为镜检阳性者),阳性率为5.47%;pPF_(14)-F-Dig 与镜检的阳性符合率为1/1,阴性符合率为94.87%。每张硝酸纤维素膜可查96份样本,故该探针具有用于大规模流行病学调查的价值。

The Plasmodium falcipaium(Pf)DNA fragment was isolatedfrom a cloned recombinant plasmid pPF14,labelled with Digoxigenin(Dig)and used as a probe(pPF14-F-Dig)to detect 274 blood specimens from theendemic area population in Hainan Province by dot hybridization.The resultsshowed that out of 274 blood samples one was positive when using microscopicesxamination and the positivity rate was 0.36 percent.Fifteen blood samplesthowed positive(including one positive specimen examined by microscopy)whenhis probe was applied to detect the 247 samples and its positivity rate was 5.47percent.The positive coincidence rate between pPF14-F-Dig and microscopicexamination was 1/1,and the negative coincidence rate was 94.87 percent.Sincea piece of nitrocellulose membrane with the size of 9 by 12 square centimetresacn be blotted 96 samples,this probe can fit the need of large-scale epidemiol-ogical surveys.