摘要
体外分离、扩增培养10例肝癌TIL细胞,用MTT法检测8例对靶细胞的杀伤活性,结果8例TIL显示对自体肿瘤细胞、SMMC—7721和K562细胞株的明显杀伤活性,并在培养30d内抗瘤活性呈现逐渐增强趋势。新鲜分离的TIL表型主要呈现CD3,培养20天后CD4+TIL细胞增加,与CD8+TIL比例为1.05。本文结果将为TIL的临床应用提供实验依据。
The present article reports our studies on isolation and expanded culture of tumor-infiltrating lymphocytes of the 10 patients with hepatocarcinoma.Killing activity of TIL on target cells was measured by MTT method in 8 cases. The results showed that hepatocarcinoma TIL presented marked cytotoxity against autogous tumor cells,SMMC-7721 and K562-cell lines.In our study,the cytotoxity of TIL against target cells increased progressively within 30 days of culture.The phenotype of freshly isolated TIL was predominetly CD3.CD4+ TIL showed increased expression,which correlated with the proportion of CD8+ TIL was 1.05.The results of this article research will provide the experimental information to clinical application.
出处
《肝胆外科杂志》
1995年第1期42-44,共3页
Journal of Hepatobiliary Surgery
关键词
肝癌
TIL
抗瘤活性
表型
liver carcinoma
TIL
Cytotoxicity
Phenotipic features