猪脾和胸腺来源免疫抑制物的对比研究

Comparing Study of Immunosuppressive Extracts from Porcine Spleen and Thymus

建立了从猪脾提制免疫抑制物的方法。通过对比研究猪脾和胸腺来源免疫抑制物的免疫药理作用,证明两者体外试验均可抑制植物凝集素诱导的人外周血淋巴细胞转化、细菌脂多糖诱导的小鼠脾细胞的分化、小鼠胸腺细胞和脾细胞的自然增殖、活化脾细胞产生白介素-2;体内试验可抑制迟发型变态反应和小鼠产生绵羊红细胞抗体;两者均无细胞毒性。两者均为与核酸结合的肽类物质,性质无明显不同。

The method of extracting immunosuppressive substances from porcine spleen was established. By the comparing study of the immunopharmacological functions of the immunosuppressive extracts from porcine spleen (SISE-p) and thymus (TISE-p), the result showed that in vitro both SISE-p and TISE-p could significantly inhibit phytohemagglutinin(PHA) induced human peripheral blood lymphocyte transformation, bacteria lipopolysaccharide(LPS) induced mouse spleen cell proliferation, the natural differentatjon of mouse spleen and thymus cells and the production of 1L-2 by activated mouse spleen cells; in vivo, both of them could suppress delayed type hypersensitivity and the production of sheep red blood cell specific antibody in mice. SISE-p and TISE-p had no cytotoxity. Biochemical property studies showed that both SISE-p and TISE-p were polypeptides combined with nucleic acids. Their properties had no significant differences.