摘要
为探讨Hu抗体在神经系统副肿瘤综合征(PNSNS)发病中的作用。用亲合免疫细胞组织化学ABC法及免疫印迹技术测定17例患者血清、脑脊液及尿液中的Hu抗体。结果显示17例患者血清中Hu抗体滴度为1∶1000至1∶64000;脑脊液(15例)中滴度为1∶10~1∶4000;尿液(5例)中滴度为1∶20~1∶200。Hu抗体识别的抗原分子量为35~40万。提示Hu抗体是与PNSNS有关的一种高度特异性自身抗体,PNSNS可能为一种自身免疫性疾病,Hu抗体在致病过程中起重要作用。
Inordertodeterminetheanti-Huantibodytitresofsera,CSFandurineinthepa-tientswithparaneoplasticsyndromesofthenervoussystem(PNSNS)andtostudythepathogenicmechanism,thepresenceofanti-Huantibodiesinsera,CSFandurinein17patientswasidentifiedwithimmunohistochemistry(ABCmethod)andwesternblotanalysis.Theresultsoftitrationsofsera,CSFandurinetotheendpointsrevealedstainingatthefinaldilutionsof1∶1000to1∶64000,1∶10to1∶4000and1∶20to1∶200respectivealy.Withwesternblotanalysistheanti-Huantibodyseraidentifiedseveralbandsofapproximately35to40kd.Theauthorsconsideredthatthesedataindicatedthattheanti-HuantibodyassociatedwithPNSNSwasahighlyspecificautoanti-body.Theanti-Hu-associatedPNSNSmightbeanautoimmunedisorder.Theproductionofanti-Huantibodiesinthecentralnervoussystemsuggestedthatitmightplayanimportantroleinthepatho-geneisofPNSNS.
出处
《中华神经科杂志》
CAS
CSCD
1996年第3期156-159,共4页
Chinese Journal of Neurology
关键词
副肿瘤综合征
Hu抗体
病理
神经系统
Paraneoplasticsyndromesofthenervoussystemanti-Huantibodies