激素敏感脂酶基因高度表达对泡沫细胞形成的作用

Effect of Hormone-sensitive Lipases Overexpression on the Formation of Chinese Hamster Ovary Cell Derived Foam Cell

中国仓鼠卵细胞在给予β－ＶＬＤＬ后，细胞内蓄积胆固醇酯，形成泡沫样细胞。以腺病毒基因转移方法书激素敏感脂酶基因转染到中国仓鼠卵细胞中，得到了来自激素敏感脂酶的胆固醇酯水解活性高度表达，此时，β－ＶＬＤＬ导致的胆固醇酯蓄积作用被阻断，提示增加胆固醇酯水解可以防止泡沫细胞形成。

A foam cell model was developed by exposure of Chinese Hamster ovary cells to 20μg/ml β-VLDL. In this foam cell model hormone-sensitive lipase(HSL) activity was overexpressed using adenovirus complexed with the rat HSL gene.Nearly 300 folds increase of intracellular cholesteryl ester hydrolase activity was achieved in transfected cells compared with the control cells. When cells overexpressing HSL were incubated with β-VLDL,cholesteryl ester levels were only 25% of those seen in control cells. Thus, HSL strongly inhibits cholesteryl ester accumulation in this foam cell model.