双重染色免疫荧光法评价选择性IgA缺乏症的B细胞成熟度

EVALUATION OF MATURITY OF B CELLS IN SELECTIVE IgA DEFICIENCY BY DOUBLE IMMUNOFLUORESCENT STUDY

选择性IgA缺乏症(SIgAD)的发病机理至今尚未十分明确。本研究力图探讨SIgAD患者B细胞分化受阻的阶段。5例患者血清IgA均少于5mg/dl。以RITC抗IgA荧光抗体和FITC抗IgD或IgM荧光抗体采用免疫荧光技术对患者外周血淋巴细胞进行双重染色。以正常成人作为对照测定B细胞的数量和表型。结果显示患者的IgA-B细胞百分数较成人低。大多数TgA-B细胞不同于成人,多同时表达表面IgM、IgD和IgA。这说明SIgAD患者的IgA-B细胞成熟受阻于分化的早期阶段。

The pathogenesis of selective IgA deficiency (SIgAD) is not clear. In an effort to determine the stage at which B cell differentiation is blocked in patients with sIgAD, peripheral-blood lymphocytes from 5 patients with serum levels of IgA less than 5 mg per deciliter were doubly stained by immunofluorescence techniques with rhodamine anti-IgA and fluorescein anti-IgD or IgM. The number and phenotype of B cells from these patients were compared with those of normal adults. IgA-B cells constituted a lower percentage of lymphocytes in patients' samples than in normal adults The majority of the IgA-B cell differed from those of normal adults', in that they expressed surface IgM and IgD as well as IgA. These results indicate that in patients with SIgAD IgA-B cell maturation is arrested at an early stage of differentiation.