摘要
本实验以赭曲霉F_(449)为出发菌株,发酵产生氨基酰化酶,其野生型菌株产酶单位为12.2μ/ml发酵液。我们首先以紫外线诱变处理2min,得到其变异株F_9,产酶16μ/ml发酵液,然后对F_9菌株的原生质体进行亚硝基胍诱变处理15min,得F_(28)菌株,产酶24.7μ/ml发酵液,将F_(28)菌株在优化培养基中发酵培养,其产酰化酶单位提高到32.4μ/ml发酵液,与此同时,提取得到粗品酰化酶,其酶活为15,000μ/g。
Aspergillus ochraceus F_(449) was used as the starting strain in the experiment.
Through fermentation, it produced aminoacylase 12.2u/mi. When A. ochraceus
F_(449) had received UV radiation for 2 minutes, mutant F_8 was obtained, which
produced aminoacylase 16u/ml. When protoplast of mutant F_9 was treated for 15
min. with NTG(N-Methyl-N'-nitro-N-nitrosoguanidine). Mutant F_(28) was obtai-
ned, which produced aminoacylase 24.1u/mi. When mutant F_(28) had been cultured
in the finest medium, the yield of aminoacylase was 32.4 u/mi. N-acethyl-DL-
phenyalanate was used as substrate, 25.9 percent of which was converted into L-
phenylalanate by aminoacylase. In the meantime, raw aminoacylase was extracted,
its activity being 15000u/g.
