冬虫夏草对小鼠红系造血机能的影响

EFFECT OF CORDYCEPS SINENSIS ON ERYTHROPOIESIS IN MOUSE BONE MARROW

用微量甲基纤维素培养方法探讨冬虫夏草结晶制剂(CS-Cr)对小鼠骨髓红系祖细胞(CFU-E和BFU-E)增殖的影响。实验结果表明,CS-Cr明显提高骨髓CFU-E和BFU-E产率、自杀率,并能对抗三尖杉酯碱对造血机能的损害。采用液体培养技术发现CS-Cr促进骨髓成纤维祖细胞(CFU-F)增殖,其产率变化与CFU-E和BFU-E产率变化一致。

The effect of cordyceps sinensis cry al (CS-Cr) stimulating proliferation of erythroid progenitor cells (CFU-E and BFU-E) in LACA mouse marrow in vivo and in vitro by methyl cellulose gel culture system is reported. The numbers of CFU-E and BFU- E were increased after 5 consecutive daily treatments with 100, 150 and 200mg/kg of CS-Cr with a peak at 150mg/kg and returned to control level in further increased doses. The cytosine arabinoside suicide technique showed a significant increase in the percentage of CFU-E or BFU E in S phase after CS-Cr treatment. pretreatment of mice with CS-Cr could protect and correctthe decrease of both CPU-E and BFU-E resulting from the cytotoxic effect of harringtoni ne. Addition of CS-Cr to culture system also promoted the generation of CFU-E and BFU-E at concentration of 150- 200μg/ml in vitro. With a lipuid culture technipue, a stimulatory effect of CS-Cr on fibroblast colony-forming unit (CPU-F)proliferation was seen in vivo and vitro. These results suggest that CS-Cr enhances erythropoiesis and CFU-F plays a role in such effect.