三尖杉酯碱对红系造血机能的影响

EFFECT OF HARRINGTONINE ON ERYTHRO POIESIS IN MOUSE BONE MARROW

用微量甲基纤维素培养方法检测三尖杉酯碱(Harringtonine,Ha)促进小鼠骨髓红系祖细胞CFU-E和BFU-E增殖的作用。结果表明,适当剂量的Ha能增强骨髓红系造血机能;如剂量过大反而起抑制作用。双盲实验也表明Ha有促进骨髓CFU-E和BFU-E增殖的作用。

An analysis w,as made of erythroid colony-forming unit(CFU-E) and erythroid burst-forming unit(BFU-E)in bone marrow of LACA mice treated with harringtonine(Ha) using a methyl cellulose gel culture system.Increased numbers of CFU-E and BFU-E were observed 24 hours after treatment with a single dose of Ha at 0.5,1.0 and 1. 5mg/kg. Then the numbers decreased while dose of Ha increased. But a stimulatory effect on the proliferation of CFU-E and BFU-E still was found after 3,6,9,12 and 15 consecutive daily treatment withl.0mg/kg of Ha. The conditioned medium from bone marrow of Ha-treated mice had no effect on the numbers of CFU-E and BFU-E. The time-response curve showed there were two peaks in numbers of CFU-E and BFU-E on days 1 and 12 after treatment. Analysis of proliferation by the cytosine arabinosis suicide technique indicated a significant increase in the percentage of CPU-Eor BFU-E in S-phase at 12 and 24 hours after treatment. The results suggest that Ha promote erythropoiesis.