应用单宁酸-氯化铁法显示小脑不同部位的微血管构筑

Observation of Microvasculature in Different Parts of Cerebellum Under the Optical Microscopy

目的 光镜下观察 6只大鼠小脑前叶、后叶及蚓部微血管构筑。方法 用单宁酸 -氯化铁法 (TAFM)媒染显示小脑内血管。结果 小脑各部位皮质、髓质动脉均来源于软脑膜动脉和小脑动脉中央支 ,软脑膜动脉分支多以直角进入小脑皮质 ,分别在分子层、蒲肯野细胞层、颗粒层形成毛细血管网 ;小脑后叶髓质中微动脉呈爪状分支 ,小叶两侧皮质深层微动脉或毛细血管可跨过髓质互相沟通。结论 TAFM显示小脑微血管构筑清晰、立体感强 。

Objective The microvasculature of the anterior lobe, posterior lobe and cerebellar vermis was observed under the optical microscope in 6 rats. Methods The tannic acid ferric chloride method (TAFM) was used to stain the vessels in cerebellum. Results The arteries within the cortex and medulla of the cerebellum originated from pia mater arteries and central branches of the cerebellar arteries, the branches of pia mater arteries mostly penetrated into the cerebellar cortex vertically and formed capillary net in the molecular layer, purkinje layer and granular layer, the arteriole in the medulla of cerebellar posterior lobe branched and formed clawed like figurations. These vessels beneath lobule cortex connected mutually by vessel anatomosis across the medulla. Conclusion The arteriole in the cerebellum can be manifested distinctly in a strong three dimension by TAFM and the vessel anatomosis in deep layer of cerebellum can be easily observed.