心脏移植排斥反应中IFN-γ mRNA表达的时间相关性实验研究

The study of the correlation between the IFN-γ mRNA expression in heart allograft and postoperative time

目的 检测心脏移植物局部IFN γmRNA在排斥反应时的动态表达情况及其与移植心自然生存时间、移植心局部细胞浸润的联系。方法 建立大鼠心脏异位移植模型 ,在移植术后第 1、3、5、7、9、11d切取移植心 ,提取总RNA ,将对照引物和目的引物在同一管中扩增 ,以目的产物和对照产物凝胶扫描像素值的比值均数图 ,直观观察排斥反应单一因素时 ,IFN γmRNA的表达水平随术后时间的延长而变化的情况。结果 排斥反应发生时 ,IFN γmRNA在心脏移植物局部的表达升高 ,表达高峰位于移植心自然停跳高峰之前 ,即出现在心脏移植术后第 5d ,其表达水平和局部的细胞浸润呈正相关。结论 IFN

Objective: This study was designed to determine the expression of IFN γ mRNA in heart allograft and its correlation to postoperative, cell infiltrating and allograft survival time. Methods: The heterotopic rat heart transplantation model was established with a simplified method, and the allografts and isografts were harvested at 1, 3,5,7,9 and 11 postoperative days. After the total RNA was prepared, the standard primer and target primer were amplified in the same tube, the average ratio of the target products pixel volume to primer's was calculated and a coordinates chart was made. Results: The expression level of IFN γ mRNA in heart allograft was elevated and its peak was on the fifth postoperative day, and prior to the heart death has a positive correlation with cell infiltrating. Conclusion: IFN γ may play on important role in postoperative rejection.