豚鼠内淋巴囊Na-K-ATP酶亚基异构体的表达

Expression of α and β subunit isoforms of Na,K-ATPase in the guinea pig endolymphatic sac

目的 研究豚鼠内淋巴囊组织中Na ,K ATP酶α、β亚基各个异构体的表达及其意义。方法 分别采用免疫组化法和原位杂交法观察豚鼠内淋巴囊组织中Na,K ATP酶α和 β亚基异构体的分布。结果 Na,K ATP酶不同的亚基异构体在豚鼠内淋巴囊上皮细胞和上皮下组织的表达有差异。内淋巴囊上皮细胞的胞膜主要表达α1 、β1 和 β2 异构体 ,且 β2 表达较 β1 表达更强 ,上皮下组织细胞的胞膜则主要表达α1 和α2 异构体 ,且α1 表达较α2 表达强 ,而上皮细胞和上皮下组织中均未见有α3异构体表达。结论 内淋巴囊组织的Na ,K ATP酶由不同亚基异构体组成 ,它们协同作用参与保持内耳内环境的稳定。

Objective To investigate the expression and significance of α and β subunit isoforms of Na,K ATPase in the guinea pig endolymphatic sac. Methods The distribution of α and β subunit isoforms of Na,K ATPase in endolymphatic sac of guinea pig was identified by immunocytochemistry and in situ hybridization. Results The expression of Na,K ATPase α and β subunit isoforms varied among different cell regions of the endolymphatic sac. Epithelial cells of the endolymphatic sac were observed to contain the α 1, β 1 and β 2 subunit isoforms, and the expression of β 2 was stronger than that of β 1. Subepithelial cells contained α 1 and α 2 subunit isoforms and the expression of α 1 was stronger than that of α 2. No expression of α 3 subunit isoform was observed in endolymphatic sac. Conclusion Na,K ATPase of endolymphatic sac consists of different α and β subunit isoforms which, working in concert, serve to maintain homeostasis in inner ear.