新城疫病毒抗人喉癌的实验研究

Study of newcastle disease virus in the treatment of human laryngeal squamous carcinoma

目的 探索喉癌生物治疗新方法。方法 用新城疫病毒 (Newcastlediseasevirus,NDV)在体外培养人喉癌细胞进行抗人喉癌细胞实验。建立人喉癌裸鼠模型 ,瘤内注射NDV ,在裸鼠体内进行抗人喉癌实验 ,对实验后的裸鼠多种组织进行病理学检查、病毒分离实验、血清酶联免疫吸附测定(enzyme linkedimmunosorbentassay,ELISA)实验。结果 NDV可在Hep 2细胞内复制、增殖 ,并引起细胞死亡 ,NDV可明显降低Hep 2细胞的独立生存与增殖能力。动物实验 ,在实验组与对照组之间经校正t检验 ,裸鼠质量t′ =2 .397(P <0 .0 5 ) ,肿瘤质量t′ =2 .85 2 (P <0 .0 5 ) ,瘤体积t′ =6 .0 5 8(P <0 .0 1) ,裸鼠质量、肿瘤质量、瘤体积的差异都有显著性 ,抑瘤率达 84.85 6 % ;病毒分离实验只有肿瘤组织中分离到病毒 ;ELISA实验组呈阳性 ;光镜和电镜下可见肿瘤细胞的坏死与凋亡及炎性细胞浸润。结论 新城疫病毒能够杀伤体外培养人喉癌细胞 ,能够抑制裸鼠体内肿瘤的生长和转移 ,且不侵犯正常组织。

Objective To explore the biological value of newcastle disease virus in the therapy of human laryngocarcinoma. Methods Nude mice model bearing laryngocarcinoma were established using human laryngeal squamous carcinoma cell line (Hep 2). Large amounts of Newcastle disease virus(NDV) were injected into the tumor. Changes in carcinoma administrated with the NDV were observed under light and electron microscopes. Isolation of NDV was attempted and sera were examined using the method of enzyme linked immunosorbent assay (ELISA). Results The difference between experimental and control groups was statistically significant, including the average weight of the mice: t ′=2.397 ( P <0.05), the average weight of the tumor tissue: t ′=2.852 ( P <0.05) and the average volume of the tumor tissue: t ′=6.058 ( P <0.01). In the experimental group, the NDV was isolated in the tumor tissue and ELISA was positive. The necrosis of the tumor cell and inflammatory cellular infiltration were found under light and electron microscopes. Conclusion These results indicated that NDV was effective in the treatment of laryngocarcinom and had no damage to normal tissue.