面神经修复中再生室内髓鞘碱性蛋白的作用初探

Preliminary study of the facial nerve regeneration in the chamber: the influence of myelin basic protein

目的 探讨外源性髓鞘碱性蛋白 (myelinbasicprotein ,MBP)在家兔面神经再生室修复中的作用。方法 将 33只家兔横断的面神经干近、远端缝于硅胶管壁上 ,形成约 12 μL大小的神经再生室。一侧为实验组 ,将MBP注入再生室内 ;对侧为对照组不注任何物质。分别在术后 4、6、8周处死动物 ,切取标本 ,在光镜下行组织形态学观察。结果 形态学分析表明术后 4周 2组再生室内再生面神经的有髓轴突直径、髓鞘厚度及长入再生室远端有髓轴突数差异无显著性 (P >0 .0 5 ) ,随着时间的延长 (术后 6、8周 ) ,MBP组较对照组再生面神经显得更为成熟 ,6周时再生轴突成熟程度差异更明显。结论 MBP有促进家兔面神经再生修复的作用 。

Objective To study the role of exogenous myelin basic protein(MBP) in neural repairment. Methods Adult New Zealand rabbits were employed in vivo preparation. A 12 μL nerve growth chamber was created by suturing the proximal and distal stumps of a transected facial never (FN) trunk into a tube. The regenerated nerves within the chambers were dissected and fixed for histological studies with light microscope at 4,6 and 8 weeks respectively following the surgery. Results Morphological analysis of nerves showed no difference between the MBP and control group in the size of the regeneration FN within the chambers, diameters of myelinated axons, thickness of myelin sheath and number of myelin axons grew into the distal end of chamber at 4 weeks. At 6 and 8 weeks after operation, the MBP group showed a more mature appearance regenerative nerve comparing to control group. Especially, the enhancement of maturation in the regeneration axons was very noticeable at 6 weeks. Conclusion The study showed that pharmacological administration of exogenous MBP within a chamber at the time of entubational nerve repair enhances regeneration of myelinated axons across the sectioned ends of FN.