青蒿琥酯对大鼠孕酮、雌二醇和蜕膜组织的影响

EFFECTS OF ARTESUNATE ON PROGESTRON ESTROGEN CONTENT AND DECIDUA IN RATS

目的 研究青蒿琥酯对妊娠大鼠血清孕酮和雌二醇含量及蜕膜组织的影响 ,以探讨其终止妊娠机理。方法 采用放射免疫分析法 (RIA)、HE染色和人蜕膜细胞体外培养等方法。结果 青蒿琥酯 40mg·kg-1·5d可使妊娠大鼠血清孕酮水平显著下降 (P <0 0 5 ) ;雌二醇有下降趋势 ,肿瘤坏死因子水平则有上升趋势 ;整体水平能损伤蜕膜组织乃至胎盘 ;对体外培养的人蜕膜细胞有直接杀伤作用 ,其LC50 为 ( 2 5± 3)mg·L-1。结论 青蒿琥酯能使血清孕酮含量下降并损伤蜕膜和胎盘而使胚胎坏死。

AIM To observe the effect of artesunate on content of progesterone, estrogen and decidua of pregnant rats and study the efficacy and mechanism of artesunate for termination of early pregnancy. METHODS Serum content of progesterone, estrogen and TNF-α were measured with RIA. The effects of artesunate on the ovary, decidua and fetus of pregnant rats were studied using histochemistry techniques. Decidual cells were estimated using cell culture. RESULTS Artesunate 40 mg·kg -1 sc on day 6-10 of gestation significantly decreased the concentration of serum progesterone in early pregnant rats; decidual cells and fetus of treated groups were found to be degenerated at d 11. Artesunate was shown to directly damage the decidual cells. Cultured human decidual cells were exposed to artesunate for 48 h, the LC 50 was found to be 25±3 mL·L -1 . CONCLUSION The damage of artesunate on decidua and placenta may be the mechanisms of its contragestational action.