诃子及其易混淆品中二种丹宁成分的高效毛细管电泳分析

ANALYSIS OF TANNINS IN FRUCTUS CHEBULAE AND ITS CONFUSION VARIETIES BY HPCE

目的 对不同来源的诃子及其易混淆品中的可水解丹宁 :诃子酸 (chebulinicacid ,I)、诃黎勒酸 (chebulagicacid ,II)进行定量分析。方法 未涂层石英毛细管 5 0 μm× 81 5cm ,有效长度 6 1 5cm ,混合缓冲溶液为 2 0mmol·L-1Na2 HPO4 + 6 0mmol·L-1硼酸 ,运行电压 2 4kV ,真空进样 5s ,检测波长 2 80nm。结果 I和II的线性范围分别为 0 0 842— 0 842和 0 0 940— 0 940mg·mL-1,相关系数分别为 0 996 6和 0 995 7,加样回收率分别为95 5 7% (RSD =4 0 % )和 94 99% (RSD =4 4% ) ,精密度试验RSD分别为 2 2 %和 1 7% ,重现性试验RSD分别为5 4%和 4 0 %。I和II的含量与诃子的品种、性状密切相关。结论 I和II可作为评价诃子药材质量的指标性成分 。

AIM To analyze the hydrolyzable tannins chebulinic acid (I) and chebulagic acid(II) in Fructus Chebulae and its confusion varieties by using high performance capillary electrophoresis (HPCE) method. METHODS Using a capillary (375 μm OD×50 μm ID; 81 5 cm×61 5 cm) and a power supply set at 24 kV, with phosphate-borate buffer containing 20 mmol·L -1 Na 2HPO 4-60 mmol·L -1 boric acid and a UV detector at 280 nm, sample solution was loaded in decompression mode at the positive end of the capillary, the loading time was 5 s. RESULTS The linear ranges of I and II were 0 0842—0 842 and 0 0940—0 940 mg·mL -1 respectively, the correlation coefficient were 0 9966 and 0 9957, the average recoveries were 95 6% (RSD=4 0%, n =5) and 95 0% (RSD=4 4%, n =5), the RSDs ( n =5) of measurement precision test were 2 2% and 1 7%, the RSDs ( n =6) of reproduction test were 5 4% and 4 0% respectively. The contents of I and II were obviously interrelated with the variety and characteristics of Fructus Chebulae, the contents of I and II in the confusion varieties of Fructus Chebulae were very low. CONCLUSION It is suitable to use I and II as the criterion in quality evaluation of Fructus Chebulae, and the HPCE method is effective for quality evaluation of the crude Fructus Chebulae.