实验性牙周炎酸性粘多糖代谢的研究

Studies on the Metabolism of Gingival Glycosaminoglycan in Experimental Periodontitis

在狗磨牙的龈沟处用丝线缝合结扎引起实验性牙周炎,再分别取下结扎7、21、60、90天的牙龈组织。把~3H葡萄糖胺作为示踪物在体外培养,制成自体放射照片标本,摄取~3H-葡萄糖胺的部位显示银色颗粒。其结果是各时期的口腔上皮,龈沟上皮、结合上皮都存在银粒子,而主要存在上皮细胞间,结缔组织内存在微量。由此可见,上皮与结缔组织相比,上皮~3H-葡萄糖胺摄取量高,代谢速度显著快。

The purpose of the study was to investigate the changes in glycosaminoglycans of the gingiva during experimental periodontitis induced by placing a silk ligature below the gingival margin of dog molars. Incorporation of ~3H-glucosamine into the gingiva was determined autoradiographically. The gingiva was collected at 7,21,60 and 90 days and cultured in vitro in the presence of ~3H-glycosamine. The autoradiographs showed a epithelial location of the silver grains in all gingival epithelia. The location was intercellular in all epithelia. The results suggest that ~3H-glucos-amine incorporation by the epithelium is greater compared with the connective tissue and markedly more rapid metabolic turnover of epithelial glycosaminoglycans.