肺癌p16、Rb基因mRNA和蛋白水平表达的定位观察

EXPRESSION OF THE p16 AND Rb GENES mRNA AND PROTEIN LEVELS IN LUNG CANCER

为研究 p16和Rb基因在蛋白水平和mRNA水平的表达及其与肺癌发生发展的关系 ,采用免疫组化和原位杂交的方法 ,对 89例肺癌手术标本进行了p16和Rb基因表达的定位观察。发现 p16基因蛋白的丢失主要发生于非小细胞肺癌 ,Rb基因蛋白的丢失主要发生于小细胞肺癌。两种基因各自在蛋白水平和mRNA水平的表达基本相符。提示p16和Rb基因的表达与肺癌的组织学类型密切相关 ,有可能作为非小细胞肺癌与小细胞肺癌基因分型诊断的分子生物学指标之一 ;在翻译水平上也存在着引起基因失活的可能机制。

The authors used immunohistochemistry and in situ hybridization to observe the expression of the p16 and Rb mRNA and protein levels in 89 cases of lung cancer. The results showed that the loss of p16 gene expression took place mainly in non small cell lung cancer, and loss of Rb gene expression mainly in small cell lung cancer. The expression of these genes on protein level fundamentally corresponded with mRNA level. It is suggested that the expression of these two genes are related to the histological type of lung cancer, and the p16 and Rb genes can be considered as one of molecular biological targets for gene classification diagnosis. There may exist a mechanism at gene translation level which leads to the inactivation of the p16 and Rb genes.