bFGF及丹参对+Gz重复暴露大鼠脑组织中iNOS mRNA表达影响

EFFECT OF BASIC FIBROBLAST GROWTH FACTOR AND RADIX SALVIAE MILTIORRHIZAE ON mRNA EXPRESSION OF INDUCED NITRIC OXIDE SYNTHASE IN BRAIN OF RATS REPEATEDLY EXPOSED TO +Gz

为了解诱导型一氧化氮合酶 (iNOS)在正加速度 (+Gz)重复暴露大鼠脑组织中表达的变化 ,探讨碱性成纤维细胞生长因子 (bFGF)及丹参对反复高 +Gz暴露致脑损伤的防护作用 ,用半定量反转录聚合酶链反应(RT -PCR)方法检测 +Gz重复暴露大鼠和bFGF、丹参处理再暴露大鼠脑组织中iNOSmRNA表达变化。结果+Gz重复暴露组可见明显的iNOSmRNA表达增高 ,而bFGF和丹参能阻断这种iNOS表达变化。表明 +Gz重复暴露可引起大鼠脑内iNOSmRNA表达变化 ,它可能在反复高 +Gz暴露致脑损伤中起重要作用 ,而bFGF和丹参对 +Gz暴露所致的脑损伤具有防护作用。

To study changes in mRNA expression of induced nitric oxide synthase (iNOS) in the brain of rat repeatedly exposed to +Gz, and to explore the prevention and treatment effect of basis fibroblast growth factor (bFGF) and radix salviae miltiorrhizae (RSM) on the brain injury induced by repeated +Gz exposures. The mRNA expression levels of iNOS in brain were measured by semi quantitative reverse transcription polymerase chain reaction (RT PCR). The results showed the iNOS mRNA expression level in brain of rats repeatedly exposed to +Gz was significantly higher than that of control, but bFGF and RSM could alleviate the expression change of iNOS. It suggested that the iNOS mRNA expression in rat brain was induced by repeated +Gz exposures and it might play an important role in the pathologic course of brain damage induced by +Gz exposures.The bFGF and RSM possessed prevention and treatment on the brain injury induced by repeated +Gz exposures.