茶多酚诱导肝癌细胞凋亡

INDUCTION OF APOPTOSIS OF HUMAN LIVER CARCINOMA CELLS BY TEA POLYPHENOLS

为进一步研究茶多酚诱导体外培养的肝癌细胞株HepG Ⅱ细胞发生凋亡的作用 ,采用MTT法、形态学观察、琼脂糖凝胶电泳和末端脱氧核苷酸转移标记法观察被茶多酚处理后的HepG Ⅱ细胞的形态学和生化等指标的变化。MTT法研究结果显示 ,当茶多酚浓度为 2 5 0 μg/ml时即可诱导HepG Ⅱ细胞凋亡 ,并与浓度呈正相关 ;当茶多酚浓度 >2 0 0 0 μg/ml时 ,抑制率增强的幅度明显减慢。透射电镜下观察到核染色质浓集呈块并可见凋亡小体 ;荧光染色在荧光显微镜下可见部分细胞核或细胞质内出现致密浓染的黄绿色块状和颗粒状荧光等凋亡细胞的形态学改变。琼脂糖凝胶电泳呈典型的DNA梯形图像。末端脱氧核苷酸转移标记法进一步证实茶多酚可诱导HepG Ⅱ细胞的凋亡。提示茶多酚可诱导体外培养的肝癌细胞株HepG Ⅱ细胞发生凋亡 ,具有抗肝癌的作用。

Tea polyphenols were tried to induce apoptosis of human cultured hepatocellular carcinoma cell line HepG Ⅱ. MTT assay, DNA agarose gel electrophoresis, transmission electron microscopy , fluorescence decoration and DNA end labeling method (Tunel) were used to identify apoptosis. Having been treated by tea polyphenols in 250μg/ml , HepG Ⅱcell apoptosis was induced. The induction of apoptosis was the dose dependent. Chromatin condensation, apoptotic body formation, fluorescence of yellow green and pellet were observed. Agarose gel electrophoresis analysis revealed DNA cleavages( DNA ladder). The results indicated that tea polyphenole could induce apoptosis of cultured human hepatocellular carcinoma cell line HepG Ⅱ. It suggested that tea polyphenols could be used to treat human liver carcinoma.