肝癌肝动脉化疗栓塞术后外周血AFP mRNA变化及意义

THE ALTERATION OF AFP mRNA CONCENTRATION IN PERIPHERAL BLOOD WITH HEPATOCELLULAR CARCINOMA DURING TRANSHEPATIC ARTERAL CHEMOTHERAPY AND EMBOLIZATION

通过对肝癌肝动脉化疗栓塞术 (TAE)治疗前后外周血AFPmRNA定量检测 ,探讨治疗前后外周血AFPmRNA定量变化规律 ,并分析其临床意义。采用分子生物学反转录竞争PCR技术 ,定量检测了 15例原发性肝细胞癌患者TAE治疗前及术后第 1、2、3、4、5、6、7、14、2 8日外周血AFPmRNA水平。结果显示TAE前后外周血AFPmRNA有不同程度的变化 ,其中术后第 3日呈最高水平 ,1周后基本恢复至术前水平 ,与术前比较 ,术后 4周 1例升高 ,8例基本相同 ,6例下降。提示TAE治疗后第 3日外周血AFPmRNA水平最高 ,临床可给予积极治疗 ,预防肝外转移的发生。

To find the rule of alterations in AFP mRNA concentration in peripheral blood in patients with hepatocellular carcinoma(HCC) before and after the treatment with transhepatic arterial chemotherapy and embolization(TAE), 15 cases of the pateints with HCC were included in this survey. The AFP mRNA concentration in peripheral blood of the patients were investigated using compatitive RT PCR technique before and 1,2,3,4, 5,6,7, 14, 28 days after TAE. The results showed that AFP mRNA concentration on the third day after TAE was the highest. It declined to the level before treatment during one week.It suggested that AFP mRNA concentration changed with a rule, and following the rule, preventive measure to distant metastasis could be instituted in clinical work.