金属硫蛋白在原发食管癌和转移淋巴结的表达及其意义

Expression of Metallothionein and Its Significance in Primary Esophageal Cancer and Their Nodal Metastasis

目的 :探讨金属硫蛋白 (MT)在食管癌和转移淋巴结组织中的表达及其与食管癌临床病理的联系。方法 :应用免疫组织化学SABC法 ,以MT和细胞角蛋白 4单克隆抗体标记 10例正常食管粘膜、 10 8例原发食管癌和 188个区域淋巴结。结果 :非典型增生的鳞状上皮MT阳性部位局限于基底和基底旁细胞 ,原发食管癌阳性率为 5 4 6 % (5 9/ 10 8) ,淋巴结转移癌MT表达情况与原发癌基本相同。MT的表达与癌组织的分级、类型和角化型鳞癌的淋巴结转移有关 (P <0 0 1,P <0 0 5 )。结论 :MT的超表达是食管粘膜上皮增生和角化型鳞癌转移的一种生物学标志 ,但其与淋巴结转移的联系则需进一步研究。

Objective:To explore the correlations of Metallothionein(MT)expression with clinicopathology in esophageal cancer and lymph node metastasis.Methods:Ten specimens normal esophageal mucosa,108 cases primary tumors and 188 regional lymph nodes were treated with immunohistochemical SABC method labelled by MT and cytokeratin 4 monoclonal antibody.Results:In dysplasia squamous epithelia positive staining with MT was confined to basal and parabasal cell.The positive staining was 54 6%(59/108) in primary esophageal cancer,and the same staining was seen in metastasis lymph node.The expression of MT was related to histological grades,patterns and lymph nodes metastasis of keratinized squamous cell carcinoma( P<0 01,P<0 05 ).Conclusion:MT over expression may be a biological marker in predicting epithelia proliferative and metastatic in keratinized squamous cell carcinoma of the esophagus.but further sdudies are needed to study the association of MT and lymph node metastasis.